Curcumin and Pgv-0 Suppress in Vitro Osteoclastogenesis and Induce Apoptosis of Osteoclast Cells

Curcumin is known could inhibit the activation of NF-kB, which is important in osteoclast differentiation and activation. The aims of this research were to conduct the effect of curcumin and Pentagamavunon-0 or PGV-0 [2,5-bis-(4’-hydroxy-3’methoxybenzylidene)-cyclopentanone], on osteoclast differentiation and apoptosis. Osteoclast differentiation was carried out using Osteoclast Differentiation Factor (ODF) induction model in Raw 264.7. The evident of osteoclast was determined microscopically using inverted microscope after TRAP staining. The apoptosis of osteoclasts were determined microscopically using confocal microscope. The results showed that PGV-0 and curcumin at the concentration of 5 μM and 10 μM, respectively inhibited osteoclast formation by 50 –80%. PGV-0 and Curcumin induced apoptosis on osteoclast cells at the concentration of 10 μM and 20 μM respectively. Those effects were dose dependent.